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KMID : 0359320070470020163
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Korean Journal of Veterinary Research
2007 Volume.47 No. 2 p.163 ~ p.167
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Prevalence for persistent infection with bovine viral diarrhea virus in Korean native calves
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Bae You-Chan
Kim Ha-Young
Park Joong-Won
Woo Gae-Hyeong
Lee O-Soo
Kang Mun-Il
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Abstract
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Bovine viral diarrhea (BVD) is very important disease in cattle industry with a worldwide distribution. Detection and elimination of persistently infected calves with bovine viral diarrhea virus (BVDV) was valuable strategy for BVD eradication because those calves were main source for transmission. We surveyed persistent infection with BVDV by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC) using whole blood and skin. Five hundred thirty nine Korean native calves were tested. Four calves (0.7%) were positive for BVDV antigen for both tests. Those calves remained positive for follow-up by RT-PCR and IHC. Therefore they were identified as persistently infected with BVDV. We confirmed that immunohistochemistry using skin biopsy samples was very useful tool to detect persistently infected calves with BVDV. As far as we know, this would be first report on persistent infection with BVDV in Korea.
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KEYWORD
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bovine viral diarrhea virus, immunohistochemistry, Korean native calf, persistent infection
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